Illumination of fly photoreceptors in the presence of the fluorescent dye Lucifer yellow initiates incorporation of the dye which stains each cell down to its synaptic terminal. Unilluminated cells do not become stained. Experiments on animals in vivo show that selected cells can be stained without loss of viability.
The presence of dye coupling and electrotonic coupling within the nucleus accumbens was examined using intracellular recording and staining in rat brain slices. In 24 of the cells examined injection of single accumbens neurons with the dye Lucifer yellow resulted in the complete labeling of more t
Iontophoretic injection of Lucifer Yellow or neurobiotin into aortic endothelium of Cx40 deficient mice showed extensive intercellular transfer of neurobiotin but not of Lucifer Yellow. In contrast intercellular spreading of Lucifer Yellow was observed in endothelium of wild type aorta.
Nov 30 2006 When using 2 mM Lucifer yellow CH 1 mg ml −1 add the dye to the internal solution of interest typically 20 ml is made up sonicate for a
Jun 27 2018 This is in stark contrast to studies from another group A previous study using injection of Lucifer yellow fluorescent dye into striatal neurons in brain slices found YAC128 MSN spine loss at 12 months of age Timed pregnancies were set up by mating wild type FVB/N female mice with YAC128 line 53 males. At E17.5 embryos were removed
with 4 Lucifer Yellow in 0.1 mol l–1 LiCl. The dye was injected by passing a hyperpolarizing current 0.2–2 nA depending on the amount of current individual electrodes would pass without blockage for 1–10 min. Following electrophysiology the brain was dissected out of the head
Nov 01 1984 By contrast at the resting junctional polarization delta V less than 0 Lucifer Yellow spread from the giant axon to the g.m.f. but not from the g.m.f. to the giant axons. These data demonstrate that dye transfer at the giant motor synapse like ionic coupling is sensitive to junctional polarization and is more marked in the high
Jan 11 2010 a Optical injection of the membrane impermeable dye Lucifer Yellow. b Optical transfection of capped GFP mRNA into primary rat hippocampal neurons i 0 min ii 30 min iii 120 min and iv 180 min. The injected neurons take up the yellow dye or express the GFP while neighbouring neurons remain unaffected. Scale bars 20 µm.
Sep 14 2019 Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis. Article. DOI 10.3791/60225. September 14th 2019 . Stefanie L. Moye 1 Blanca Diaz Castro 1 Mohitkumar R. Gangwani 1 Baljit S. Khakh 1 2. 1 Department of Physiology David Geffen School of Medicine University of California Los Angeles 2 Department of Neurobiology
Cells were loaded by fluid phase uptake with a mixture of the Lucifer Yellow dextran LY dex a D2O sensitive dye and a macropinosome like spacious vacuole which does not de D2O insensitive control dye Alexa fluor 546 dextran AF546 dex .
Aug 31 2019 a Larva before injection on the right A spot of Lucifer Yellow on filter paper b 10 min and c 1.5 h after injection of Lucifer Yellow solution 5 µL 1
following of the dye from the moment of injection and use of the simple vein system allowed an unhindered and precise assessment of the cell to cell route of dye transfer. Disodium fluorescein and Lucifer Yellow CH injected into mesophyll or mestome sheath cells readily moved to the sieve tube. This symplastic dye transfer from mesophyll to
Gap junction coupling was analyzed by dye transfer experiments with Lucifer Yellow LY lithium salt Biotium . Cells grown on coverslips were transferred into a perfusion chamber filled with 500 μL of a bath solution 140 mM NaCl 5 mM KCl 10 mM HEPES 1 mM MgCl 2 10 mM glucose 2 mM CaCl 2 at pH 7.4 and 295 mosmol/L .
Dye injections imaging and analysis. For anatomical analysis the dis sected CNS was mounted dorsal side up on a poly lysine coated slide. The preparation was immersed in saline and viewed with a 40 water immersion lens. The axons of the GFs are identified in the connective using differential interference contrast optics. A glass electrode
Dec 07 1998 In contrast in white matter the dye was restricted to the injected cell N = 63 for Lucifer Yellow injection only N = 11 for LY and Neurobiotin indicating a lack of functional coupling. Immunolabeling of Cx32 in mature oligodendrocytes of white matter revealed that the gap junction protein is localized on the cell bodies and abaxonal
with 5–10 Lucifer yellow with a 0.2 to 3.0 nA negative current for 10–30 min Fig. 2C according to a standard iontophoretic method Cash et al. 1992 . Immunocytochemistry. After dye injection the larva was fixed again for 30 min with 4 paraformaldehyde and reacted with anti Lucifer yellow antibodies dilution 1 100 Molecular Probes
May 04 2021 Strategies to increase the proportion of neural stem cells that differentiate into neurons are vital for therapy of neurodegenerative disorders. In vitro the extracellular matrix composition and topography have been found to be important factors in stem cell differentiation. We have developed a novel artificial extracellular matrix aECM formed by attaching gold
May 01 1993 In contrast little diffusion of Lucifer yellow was observed in SaOS 2 monolayers 1.4 / 1.8 coupled cells per injection n = 100 . Dye diffusion was inhibited by octanol 3.8 mM an inhibitor of gap junctional communication. All of the osteoblastic cells expressed mRNA for connexin43 and connexin45 but not for connexins 26 32 37 40 or 46.
FIG. 3. A and C Phase contrast micrographs. B andD Fluo rescentmicrographsofsamecells. Micrographsweretaken 1 2min after injection of Lucifer yellow. X300. Dye injected into single SKHeplcells brightest cell in eachfield is confinedtothe injected cell in controls A B but spreads rapidly to adjacent cells in transfected colonies C D .
Lucifer Yellow CH dilithium salt Code SCA76947 / CAS No 67769 47 5 yellow neurons fluorescent methods results transport injected protein method cortex fluorescence injection system fluorescent dye study molecules studies vitro sample neuronal target activity brain cortical staining. Tetrodotoxin Bio X
Lucifer Yellow was used as a control since this bright fluorescent dye is insensitive to changes in pH or the cytosolic Ca 2 concentration. The application of injection currents up to −1 nA via the third barrel resulted in loading of cells with fluorescent dyes in approximately 1 min Figure 1 A Supplemental Movie 1 .
Following the recording ofelectrophysiological characteristics ofthe neurone injection of an intracellular dyewasmadebyelectrophoresis usingoutwardcurrentpulsesfor HRPandinward pulsesfor Lucifer Yellow bothatapulse frequencyof0 5 Hz. Thepulse durationwas500msfor HRPand 1 s for Lucifer Yellow and the current intensity was 1 5nA that is as
Lucifer yellow. Lucifer yellow CH was obtained from Sigma and working solutions 0.16 1.0 mg/ml were prepared directly in distilled water or a solution containing 1 mM Hepes pH 7.0 1 mM KCl and 0.04 mM CaCl2. The dye solution was filtered through Acrodiscs 0.2 pore size Gelman Sciences Ann Arbor Mich. USA immediately after preparation
Each practitioner performed a series of 10 injections into a simulated patient IV setup Figure 1 . Figure 1. Injection study setup with Lucifer Yellow contrast dye syringe IV tubing stopcock and 10 mL collection vial.
Feb 09 1996 In the same way we injected enzymes and their substrates in a plant cell without loss of their bioactivity unpublished data . In this study we intended to inject a fluorescence dye in a cell and then translocate it to a neighboring cell according to the strategy mentioned above. Lucifer yellow CH LY was selected as the demonstrative compound.
Oct 31 2014 Downregulation of endothelial connexins has been shown to result in impaired angiogenesis. Isoprenaline is known to upregulate Cx43 in cardiomyocytes. Effects of isoprenaline on endothelial connexins are unknown. We wanted to investigate whether isoprenaline might induce upregulation of connexins Cx37 Cx40 or Cx43 in human
Jan 31 2013 However in the case of diffusing lucifer yellow which is a highly hydrophilic solute with a K OW of −6.846 the main permeation should be through aqueous pores and/or shunts Chan et al. 2005 . Thus the fluorescence signal of lucifer yellow is intense in the outermost layer of the stratum corneum hair follicles and sweat ducts.
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